Novel phenolic compound from Southern Ocean microalgae Chlorella sp. PR-1 and its antibacterial activity

Authors

  • Pratibha Gupta Department of Bio-Engineering, Birla Institute of Technology, Mesra, Jharkhand, India https://orcid.org/0000-0003-2708-3267
  • Aparna Banerjee Centro de Investigación de Estudios Avanzados del Maule (CIEAM), Vicerrectoría de Investigación y Postgrado, Universidad Católica del Maule, Talca, Chile https://orcid.org/0000-0002-5061-4903
  • Alexis Castillo Centro de Investigación de Estudios Avanzados del Maule (CIEAM), Vicerrectoría de Investigación y Postgrado, Universidad Católica del Maule, Talca, Chile https://orcid.org/0000-0003-3342-2632
  • Rajib Bandopadhyay UGC-Center of Advanced study, Department of Botany, The University of Burdwan, Golapbag, Purba Bardhaman-713104, West Bengal, India https://orcid.org/0000-0002-8318-5631

Keywords:

Southern Ocean, microalgae, phenolic compound, antibacterial activity

Abstract

Marine microalgae has been attracting the researcher’s attention for centuries. Development on microalgal research is majorly favoured by its medicinal, pharmaceutical or cosmeceutical properties. The advancement in the investigation related to microalgal products have been concentrated in the coastal zones because of the greater supply of raw material. The Southern Ocean, highly productive and relatively poorly studied ecosystem, constitutes approximately 10% of the global volume of the oceans. In this study, marine microalgae Chlorella sp. PR-1 was isolated from the Southern Ocean (Indian Sector) for the identification of bioactive antibacterial compounds. The antimicrobial activity of this microalgal extracts was evaluated against three gram positive (Staphylococcus aureus, Bacillus licheniformis, and Bacillus subtilis) and three gram negative bacteria (Pseudomonas aeruginosa, Salmonella typhimurium, and Escherichia coli). The extract showing antibacterial activity was further purified by thin layer and column chromatography. The antimicrobial activity was again evaluated and confirmed with the purified fraction against the same bacteria. The identification of the functional groups in the purified fraction was performed by infrared spectroscopic analysis. Based on gas chromatographic and mass spectroscopic analysis, the principle bioactive compound was proved to be 2,4-bis (1,1-dimethylethyl)- phenol. Thus, the bioactive compound isolated from marine microalga of Southern Ocean origin may be a novel alternative source of antibacterials in the future.

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Published

2021-06-30

How to Cite

(1)
Gupta, P.; Banerjee, A.; Castillo, A.; Bandopadhyay, R. Novel Phenolic Compound from Southern Ocean Microalgae Chlorella Sp. PR-1 and Its Antibacterial Activity. Gayana Bot. 2021, 78, 29-37.

Issue

Section

ORIGINAL ARTICLES