Effect of scarification and stratification on the in vitro germination of Aristotelia chilensis (Molina) Stuntz

Authors

  • Mario Rodríguez Beraud Universidad Católica de Temuco, Temuco, Chile
  • Jocelyne Tampe Pérez Universidad de La Frontera, Temuco, Chile
  • Nelson Hormazábal Vásquez Universidad Católica de Temuco, Temuco, Chile
  • Ximena Araneda Durán Universidad Católica de Temuco, Temuco, Chile
  • Ricardo Tighe Neira Universidad Católica de Temuco, Temuco, Chile
  • Paz Cárcamo-Fincheira Universidad Católica de Temuco, Temuco, Chile

Keywords:

dormancy, maqui, propagation, seeds

Abstract

Two in vitro assays were performing in order to improve Aristotelia chilensis germination rate. Scarification was tested in the first assay and compare to a control. In the second assay, cold stratification was applied during 2, 4, 6 and 8 weeks at 4 °C in darkness, plus an unstratified control. Seeds were germinated on Petri dish containing water and agar (WA), and incubated for 60 days in a controlled atmosphere at 25 ± 2 °C using cold white fluorescent light tubes (50 μmol m-2 s-1 light intensity), under a photoperiod of 16 h light and 8 h of darkness. All treatments achieved higher average germination rates than the control. The highest germination rate was obtained with scarification (Gmáx, 92%) though the fastest growth was achieved with 8 weeks of stratification, reaching the lowest average germination time of 18 days. The results demonstrate that A. chilensis seeds present a moderate level of primary dormancy, both exogenous and endogenous.

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Published

2017-12-30

How to Cite

(1)
Rodríguez Beraud, M. .; Tampe Pérez, J.; Hormazábal Vásquez, N.; Araneda Durán , X.; Tighe Neira, R.; Cárcamo-Fincheira, P. Effect of Scarification and Stratification on the in Vitro Germination of Aristotelia Chilensis (Molina) Stuntz. Gayana Bot. 2017, 74, 282-287.

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Section

ORIGINAL ARTICLES

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