Success and failures in the inoculation of five introduced trees in Chile with Tuber magnatum Pico: First advances for the domestication of the white truffle in South America


  • Guillermo Pereira Departamento de Ciencias y Tecnología Vegetal, Universidad de Concepción, Escuela de Ciencias y Tecnologías, Los Ángeles, Chile
  • Götz Palfner Departamento de Botánica, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Concepción, Chile
  • Laura M. Suz Comparative Plant and Fungal Biology, Royal Botanic Gardens Kew, Richmond Surrey, UK
  • Patricio Sandoval Departamento de Ciencias y Tecnología Vegetal, Universidad de Concepción, Escuela de Ciencias y Tecnologías, Los Ángeles, Chile.
  • Ricardo Ramírez Andean Truffles SPA, Parcela 5 Santa Ana, Mulchén, Chile
  • Daniel Chávez Centro de Investigación en Micorrizas y Sustentabilidad Agroambiental (CIMYSA), Universidad de La Frontera, Temuco, Chile
  • Cristian Atala Pontificia Universidad Catolica de Chile


Corylus avellana, ITS-rDNA, mycorrhizal synthesis, Quercus cerris, Quercus robur, white truffles


Truffles are highly demanded edible fungi. They belong to the genus Tuber and have very high economic value, with Tuber magnatum being one of the most expensive and gastronomically used. This study evaluates the potential of five introduce tree species as mycorrhizal hosts for T. magnatum in Chile. Seeds from Quercus cerris, Q. robur and Corylus avellana were harvested from adult trees. Cuttings from Populus nigra and Salix caprea were harvested in the field and asexually propagated under greenhouse conditions. After two months, they were transplanted to 260 cc pots containing sterilized composted pine bark. A spore suspension (106 spores/plant) of T. magnatum was injected directly into plant roots. Three months after inoculation we observed spore germination and the presence of mycelium around the roots in some seedlings. After seven months, we observed mycorrhizae from T. magnatum only in Q. cerris, Q. robur and C. avellana, characterized by their epidermoid mantle and awl-shaped, bristle-like cystidia. The identification the mycorrhizal structures was confirmed by sequencing of the nuclear ITS- rDNA region. This study provide the first advances for the domestication of this highly valuable truffle in Chile and South America and the successful mycorrhized plants could be used in further field assays. Some Quercus, Populus and Salix species have been used as ornamental plants and are naturalized in Chile for over 100 years. Additionally, hazelnut (Corylus avellana) is currently grown in the country under intensive silviculture. These tree species could act as a non-intentional host for truffles, dispersed from production sites.



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Author Biography

Cristian Atala, Pontificia Universidad Catolica de Chile

Biologo, Universidad de Chile

Dr. Ciencias Biologicas area Botanica, Universidad de Concepción




How to Cite

Pereira, G.; Palfner, G.; Suz, L. M.; Sandoval, P.; Ramírez, R.; Chávez, D.; Atala, C. Success and Failures in the Inoculation of Five Introduced Trees in Chile With Tuber Magnatum Pico: First Advances for the Domestication of the White Truffle in South America. Gayana Bot. 2021, 78, 19-28.




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