TY - JOUR AU - Pereira, Guillermo AU - Palfner, Götz AU - Suz, Laura M. AU - Sandoval, Patricio AU - Ramírez, Ricardo AU - Chávez, Daniel AU - Atala, Cristian PY - 2021/06/30 Y2 - 2024/03/28 TI - Success and failures in the inoculation of five introduced trees in Chile with Tuber magnatum Pico: First advances for the domestication of the white truffle in South America JF - Gayana Botanica JA - Gayana Bot. VL - 78 IS - 1 SE - ORIGINAL ARTICLES DO - UR - https://gayanabotanica.cl/index.php/gb/article/view/212 SP - 19-28 AB - <p>Truffles are highly demanded edible fungi. They belong to the genus <em>Tuber </em>and have very high economic value, with <em>Tuber magnatum </em>being one of the most expensive and gastronomically used. This study evaluates the potential of five introduce tree species as mycorrhizal hosts for <em>T. magnatum </em>in Chile. Seeds from <em>Quercus cerris, Q. robur </em>and <em>Corylus avellana </em>were harvested from adult trees. Cuttings from <em>Populus nigra </em>and <em>Salix caprea </em>were harvested in the field and asexually propagated under greenhouse conditions. After two months, they were transplanted to 260 cc pots containing sterilized composted pine bark. A spore suspension (106 spores/plant) of <em>T. magnatum </em>was injected directly into plant roots. Three months after inoculation we observed spore germination and the presence of mycelium around the roots in some seedlings. After seven months, we observed mycorrhizae from <em>T. magnatum </em>only in <em>Q. cerris</em>, <em>Q. robur </em>and <em>C. avellana</em>, characterized by their epidermoid mantle and awl-shaped, bristle-like cystidia. The identification the mycorrhizal structures was confirmed by sequencing of the nuclear ITS- rDNA region. This study provide the first advances for the domestication of this highly valuable truffle in Chile and South America and the successful mycorrhized plants could be used in further field assays. Some <em>Quercus, Populus </em>and <em>Salix </em>species have been used as ornamental plants and are naturalized in Chile for over 100 years. Additionally, hazelnut (<em>Corylus avellana</em>) is currently grown in the country under intensive silviculture. These tree species could act as a non-intentional host for truffles, dispersed from production sites.</p><p><em> </em></p> ER -